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Product Name
Anti-AMIGO2 / AMIGO-2 / alivin 1 antibody
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Description
Rabbit polyclonal to AMIGO2 / AMIGO-2 / alivin 1
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Tested applications
ELISA, IHC-P
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Species reactivity
Human AMIGO2 / AMIGO-2 / alivin 1
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Alternative names
Ali1 antibody; AMIGO-2 antibody; AI415330 antibody; AW208913 antibody; ALI1 antibody; DEGA antibody; AMIGO-2 antibody; adhesion molecule with Ig like domain 2 antibody; adhesion molecule with Ig-like domain 2 antibody; AI415330 antibody; ALI1 antibody; Ali1 antibody; alivin 1 antibody; alivin 1 antibody; Amigo2 antibody; AMIGO2 antibody; AMIGO-2 antibody; AMIGO-2 antibody; amphoterin induced gene 2 antibody; amphoterin induced gene and ORF 2 antibody; amphoterin-induced protein 2 antibody; amphoterin-induced protein 2 antibody; AW208913 antibody; DEGA antibody; differentially expressed in gastric adenocarcinoma antibody; differentially expressed in gastric adenocarcinomas antibody; transmembrane protein AMIGO2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human AMIGO2 / AMIGO-2 / alivin 1 (rh AMIGO2 / AMIGO-2 / alivin 1; NP_862830.1; Met1-Thr398). AMIGO2 / AMIGO-2 / alivin 1 specific IgG was purified by Human AMIGO2 / AMIGO-2 / alivin 1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human AMIGO2 / AMIGO-2 / alivin 1. The detection limit for Human AMIGO2 / AMIGO-2 / alivin 1 is 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
AMIGO2 / AMIGO-2 / alivin 1 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human AMIGO2 in human breast carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
AMIGO2 / AMIGO-2 / alivin 1 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human AMIGO2 in human gastric cancer with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
AMIGO2 / AMIGO-2 / alivin 1 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human AMIGO2 in human breast with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
AMIGO2 contains Ig-like C2-type (immunoglobulin-like) domain, 6 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. It belongs to the immunoglobulin superfamily, AMIGO family. AMIGO2 may mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. It is required for depolarization-dependent survival of cultured cerebellar granule neurons. AMIGO2 may also contribute to signal transduction through its intracellular domain. It may play a role in the tumorigenesis of a subset of gastric adenocarcinomas. AMIGO2 is highly expressed in breast, ovary, cervix, and uterus.
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References
- Rouhiainen A, et al. (2003) AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats. J Cell Biol. 160:963-73.
- Kikkawa Y, et al. (2003) Alivin 1, a novel neuronal activity-dependent gene, inhibits apoptosis and promotes survival of cerebellar granule neurons. J Neurosci. 23:5887-96.
- Bassi R, et al. (2004) DEGA/AMIGO-2, a leucine-rich repeat family member, differentially expressed in human gastric adenocarcinoma: effects on ploidy, chromosomal stability, cell adhesion/migration and tumorigenicity. Oncogene 23:5056-67.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"