-
Product Name
Anti-Alkaline Phosphatase / ALPL antibody
- Documents
-
Description
Rabbit polyclonal to Alkaline Phosphatase / ALPL
-
Tested applications
IHC-P
-
Species reactivity
Human ALPL
-
Alternative names
HOPS antibody; TNAP antibody; APTNAP antibody; TNSALP antibody; AP-TNAP antibody; Akp2 antibody; TNAP antibody; Akp-2 antibody; APTNAP antibody; TNSALP antibody; Akp2 antibody; Akp-2 antibody; Alkaline Phosphatase antibody; Alkaline Phosphatase antibody; Alpl antibody; ALPL antibody; AP-TNAP antibody; AP-TNAP antibody; FLJ40094 antibody; FLJ93059 antibody; HOPS antibody; MGC161443 antibody; MGC167935 antibody; RP23-277N22.4 antibody; TNAP antibody; TNAP antibody; TNSALP antibody; TNSALP antibody
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the human ALPL , and purified by antigen affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
IHC-P: 0.1-1 μg/mL
-
Validations
ALPL Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human ALPL in human adrenal gland with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
-
Background
Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.
-
References
- Brun-Heath I, et al. (2011) Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues. Cell Tissue Res. 343(3): 521-36.
- Whyte MP, et al. (1995) Alkaline phosphatase: placental and tissue-non-specific isoenzymes hydrolyze phosphoethanolamine, inorganic pyrophosphate, and pyridoxal 5-phosphate. J Clin Invest. 95: 1440-5.
- Whyte MP. (1994) Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization. Endocrinol Rev. 4: 439-61.
- Weinreb M, et al. (1990) Different pattern of alkaline phosphatase, osteopontin and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res. 5: 831-42.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"