Product Name

Anti-ALK-2 / ACVR1 antibody

Documents

Description

Rabbit polyclonal to ALK-2 / ACVR1

Tested applications

ELISA, IHC-P

Species reactivity

Human ALK-2 / ACVR1

Alternative names

ACTRI antibody; FOP antibody; FOP antibody; ALK2 antibody; SKR1 antibody; TSRI antibody; ACTRI antibody; ACVR1A antibody; ACVRLK2 antibody; ALK2 antibody; Acvr antibody; Alk8 antibody; SKR1 antibody; Alk-2 antibody; Tsk7L antibody; ActR-I antibody; ActRIA antibody; Acvrlk2 antibody; D330013D15Rik antibody; ActR-I antibody; ActRIA antibody; Acvr antibody; ACVR1 antibody; Acvr1 antibody; ACVR1A antibody; Acvrlk2 antibody; ACVRLK2 antibody; ALK2 antibody; ALK2 antibody; Alk-2 antibody; Alk8 antibody; D330013D15Rik antibody; RP23-100B18.4 antibody; SKR1 antibody; SKR1 antibody; Tsk7L antibody; TSRI antibody

Immunogen

Human ALK-2 / ACVR1 (His Tag) recombinant protein

Isotype

Rabbit IgG

Preparation

Produced in rabbits immunized with purified, recombinant Human ALK-2 / ACVR1 (rh ALK-2 / ACVR1; Q04771; Met1-Val124). ALK-2 / ACVR1 specific IgG was purified by Human ALK-2 / ACVR1 affinity chromatography.

Clonality

Polyclonal

Formulation

0.2 μm filtered solution in PBS

Storage instructions

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Applications

ELISA:0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ALK-2 / ACVR1. The detection limit for Human ALK-2 / ACVR1 is < 0.039 ng/well.

IHC-P: 0.1-2 μg/ml

Validations

ALK-2 / ACVR1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

Background

ALK-2, also termed as ACVR1, was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presenting in the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro, and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Overexpression of ALK-2, but not of ALK-1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domain threonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP).

References

Armes NA,et al. (1997) The ALK-2 and ALK-4 activin receptors transduce distinct mesoderm-inducing signals during early Xenopus development but do not co-operate to establish thresholds. Development 124(19): 3797-804.
Armes NA, et al. (1999) A short loop on the ALK-2 and ALK-4 activin receptors regulates signaling specificity but cannot account for all their effects on early Xenopus development. J Biol Chem. 274(12):7929-35.
Kawai S, et al. (2000) Mouse smad8 phosphorylation downstream of BMP receptors ALK-2, ALK-3, and ALK-6 induces its association with Smad4 and transcriptional activity.Biochem Biophys Res Commun. 271(3):682-7.
Deng Y, et al. (2009) Efficient highly selective synthesis of methyl 2-(ethynyl)alk-2(E)-enoates and 2-(1'-chlorovinyl)alk-2(Z)-enoates from 2-(methoxycarbonyl)-2,3-allenols. Organic letters 11(10):2169-72.

Anti-ALK-2 / ACVR1 antibody

Product Information

Related Products / Services