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Product Name
Anti-Aire antibody
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Description
Rabbit polyclonal antibody to Aire
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Tested applications
WB, ICC, IHC-P
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Species reactivity
Mouse
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was peptide
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:2,000
ICC: 1:500
IHC-P: 1:100
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Validations
Fig1: Western blot analysis on 293 cell (A) transfected and (B) none transfected with AIRE gene using anti-AIRE polyclonal antibody.
Fig2: Immunohistochemistry- staining AIRE in mouse thymus by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Fig3: AIRE staining (red) in mouse embryonic stem cells (D3) by immunocytochemistry, nuclear staining with DAPI (blue) and SSEA-1 staining (green).
- Background
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References
- Isolation and characterization of the mouse Aire gene.Mittaz L., Rossier C., Heino M., Petersen P., Krohn K.J.E., Gos A., Morris M.A., Kudoh J., Shimizu N., Antonarakis S.E., Scott H.S.Biochem. Biophys. Res. Commun. 255:483-490(1999)
- Aire employs a histone-binding module to mediate immunological tolerance, linking chromatin regulation with organ-specific autoimmunity.Koh A.S., Kuo A.J., Park S.Y., Cheung P., Abramson J., Bua D., Carney D., Shoelson S.E., Gozani O., Kingston R.E., Benoist C., Mathis D.Proc. Natl. Acad. Sci. U.S.A. 105:15878-15883(2008)
- The autoimmune regulator (AIRE) is a DNA-binding protein.Kumar P.G., Laloraya M., Wang C.-Y., Ruan Q.-G., Davoodi-Semiromi A., Kao K.-J., She J.-X.J. Biol. Chem. 276:41357-41364(2001)
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