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Product Name
Anti-ACO2 / Aconitase 2 antibody
- Documents
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Description
Rabbit polyclonal to ACO2 / Aconitase 2
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Mouse ACO2 / Aconitase 2
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Alternative names
ICRD antibody; ACONM antibody; Aco3 antibody; Aco-2 antibody; D10Wsu183e antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse ACO2 / Aconitase 2 (rh ACO2 / Aconitase 2; Q99KI0; Gln28-Gln780). ACO2 / Aconitase 2 specific IgG was purified by Mouse ACO2 / Aconitase 2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ACO2 / Aconitase 2. The detection limit for Mouse ACO2 / Aconitase 2 is < 0.039 ng/well.
IHC-P: 0.05-1 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
ACO2 / Aconitase 2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
ACO2 / Aconitase 2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse ACO2 in mouse heart with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
ACO2 / Aconitase 2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse ACO2 in mouse kidney with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
ACO2 / Aconitase 2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse ACO2 in mouse skeletal muscle with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
ACO2 / Aconitase 2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
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References
- Robbins AH, et al. (1989) The structure of aconitase. Proteins. 5 (4): 289-312.
- Lauble H, et al. (1992) Crystal structures of aconitase with isocitrate and nitroisocitrate bound. Biochemistry. 31 (10): 2735-48.
- Robbins AH, et al. (1989) Structure of activated aconitase: formation of the 4Fe-4S cluster in the crystal. Proc Natl Acad Sci. 86 (10): 3639-43.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"