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Product Name
Anti-Acetyl-Histone H3 (Lys27) Rabbit antibody
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Description
Acetyl-Histone H3 (Lys27) Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP, ChIP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Validations
Western blot detection of Histone H3 (acetyl-Lys27) in Hela,A549,HL-60 cell lysates using Histone H3 (acetyl-Lys27) Rabbit mAb(1:1000 diluted).Predicted band size:15kDa.Observed band size:17kDa.
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Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
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