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Product Name
Anti-ABHD14B antibody
- Documents
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Description
Rabbit monoclonal to ABHD14B
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Tested applications
WB, IP
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Species reactivity
Human ABHD14B
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Alternative names
CIB antibody; HEL-S-299 antibody; 1810013B01Rik antibody; 1810013B01Rik antibody; abhydrolase domain-containing protein 14B antibody; abhydrolase domain-containing protein 14B antibody; CCG1-interacting factor B antibody; CCG1-interacting factor B antibody; cell cycle gene 1-interacting factor B antibody; CIB antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (rh ABHD14B; Q96IU4-1; Met1-Gln210).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% treha
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-15 μg/ml
IP: 1-4 μg/mg of lysate
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Validations
ABHD14B Antibody, Rabbit MAb, Western blot
ABHD14B Antibody, Rabbit MAb, Immunoprecipitation
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Background
ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.
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References
- Mehrle A, et al. (2006) The LIFEdb database in 2006. Nucleic Acids Res. 34:D415-8.
- Wan D, et al. (2004) Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc Natl Acad Sci. 101(44):15724-9.
- Wiemann S, et al. (2004) From ORFeome to biology: a functional genomics pipeline. Genome Res. 14 (10B):2136-44.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"