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Product Name
Anti-Abcb1a antibody
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Description
Rabbit polyclonal antibody to Abcb1a
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Tested applications
WB, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
P antibody; P- antibody; Evi antibody; Mdr antibody; Pgp antibody; Pgy antibody; Mdr3 antibody; P-gp antibody; Pgy- antibody; Pgy3 antibody; mdr- antibody; Abcb4 antibody; Evi32 antibody; Mdr1a antibody; Pgy-3 antibody; mdr-3 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human mdr1 21-100/1272
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
FC:1μg/Test
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Validations
Fig1: Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice).; Primary Antibody (green line): Rabbit Anti-MDR1 antibody ,Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
Fig2: Sample: Liver (mouse) Lysate at 40 ug; Primary: Anti- MDR1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 141kD; Observed band size: 150kD
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"