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Product Name
Anti-2B4 / CD244 antibody
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Description
Rabbit monoclonal to 2B4 / CD244
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Tested applications
WB, IP
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Species reactivity
Human 2B4 / CD244
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Alternative names
Ly90 antibody; NAIL antibody; Nmrk antibody; 2B4 antibody; 2B4 antibody; C9.1 antibody; Cd244 antibody; CD244 antibody; F730046O15Rik antibody; Ly90 antibody; NAIL antibody; NAIL antibody; NKR2B4 antibody; NKR2B4 antibody; Nmrk antibody; Nmrk] antibody; SLAMF4 antibody; SLAMF4 antibody; NKR2B4 antibody; SLAMF4 antibody; F730046O15Rik antibody; 2B4 antibody; NAIL antibody; Nmrk antibody; NKR2B4 antibody; SLAMF4 antibody; 2B4 antibody; C9.1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human 2B4 / CD244 (rh 2B4 / CD244; NP_057466.1; Met1-Arg221).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
2B4 / CD244 Antibody, Rabbit MAb, Western blot
2B4 / CD244 Antibody, Rabbit MAb, Immunoprecipitation
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Background
The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.
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References
- Nakajima H, et al. (2000) 2B4: an NK cell activating receptor with unique specificity and signal transduction mechanism. Hum Immunol. 61(1): 39-43.
- Chuang SS, et al. (2001) 2B4 (CD244)-mediated activation of cytotoxicity and IFN-gamma release in human NK cells involves distinct pathways. J Immunol. 167(11): 6210-6.
- McNerney ME, et al. (2005) 2B4 (CD244) is a non-MHC binding receptor with multiple functions on natural killer cells and CD8+ T cells. Mol Immunol. 42(4): 489-94.
- Mathew SO, et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur J Immunol. 39(6): 1632-41.
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