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Product Name
ANO2 Polyclonal Antibody
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Description
Polyclonal antibody to ANO2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ANO2 antibody; C12orf3 antibody; TMEM16B antibody; anoctamin-2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ANO2 (NP_001265526.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - ANO2 Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using ANO2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 20s.
Immunohistochemistry - ANO2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat retina using ANO2 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - ANO2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse retina using ANO2 antibody at dilution of 1:200 (40x lens).
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Background
Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"