ANO1 Polyclonal Antibody
Polyclonal antibody to ANO1
Human, Mouse, Rat
ANO1 antibody; DOG1 antibody; ORAOV2 antibody; TAOS2 antibody; TMEM16A antibody; anoctamin-1 antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 628-731 of human ANO1 (NP_060513.5).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:1000 - 1:2000
IF 1:100 - 1:200
Western blot - ANO1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ANO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunofluorescence - ANO1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using ANO1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"