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Product Name
Annexin V antibody
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Description
Annexin V Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, HepG2 cells, human heart tissue, human lung tissue, human placenta tissue, human skeletal muscle tissue, NIH/3T3 cells. Positive IHC detected in human spleen tissue, human lung cancer tissue, human placenta tissue. Positive IF detected in HeLa cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 36 kDa
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Tested applications
ELISA, WB, IP, IHC, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Anchorin CII antibody; Annexin 5 antibody; annexin A5 antibody; Annexin V antibody; ANX5 antibody; ANXA5 antibody; Calphobindin I antibody; CBP I antibody; Endonexin II antibody; ENX2 antibody; LipOCortin V antibody; PAP I antibody; PP4 antibody; Thromboplastin inhibitor antibody; VAC alpha antibody; Vascular anticoagulant alpha antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Annexin V recombinant protein (Accession Number: NM_001154). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108096(ANXA5 antibody) at dilution of 1:800
IP Result of anti-ANXA5 (IP:Catalog No:108096, 3ug; Detection:Catalog No:108096 1:500) with HeLa cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:108096(ANXA5 Antibody) at dilution of 1:400 (under 10x lens)
Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:108096(ANXA5 Antibody) at dilution of 1:400 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:108096(ANXA5 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug ANXA5 antibody (Catalog No:108096, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.
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References
- Cui Y, Zhang D, Jia Q, Li T, Zhang W, Han J. Proteomic and tissue array profiling identifies elevated hypoxia-regulated proteins in pancreatic ductal adenocarcinoma. Cancer investigation. 27(7):747-55. 2009.
- Cristobo I, Brea D, Blanco M. Usefulness of material recovered from distal embolic protection devices after carotid angioplasty for proteomic studies. Journal of vascular and interventional radiology : JVIR. 23(6):818-24. 2012.
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