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Product Name
Annexin V antibody
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Description
Annexin V Mouse Monoclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, K-562 cells. Positive FC detected in HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 36 kDa
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Tested applications
ELISA, WB, IF, FC
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Species reactivity
Human; other species not tested.
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Alternative names
Anchorin CII antibody; Annexin 5 antibody; annexin A5 antibody; Annexin V antibody; ANX5 antibody; ANXA5 antibody; Calphobindin I antibody; CBP I antibody; Endonexin II antibody; ENX2 antibody; LipOCortin V antibody; PAP I antibody; PP4 antibody; Thromboplastin inhibitor antibody; VAC alpha antibody; Vascular anticoagulant alpha antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Annexin V recombinant protein (Accession Number: NM_001154). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107058(ANXA5 Antibody) at dilution of 1:1000
Immunofluorescent analysis of HeLa cells using Catalog No:107058(ANXA5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug ANXA5 antibody (Catalog No:107058, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.
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Background
Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.
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