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Annexin IV antibody

Cat.#: 108095

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Product Information

  • Product Name
    Annexin IV antibody
  • Documents
  • Description
    Annexin IV Rabbit Polyclonal antibody. Positive IHC detected in human stomach tissue. Positive WB detected in RAW 264.7 cells, HEK-293 cells, HeLa cells, human placenta tissue. Observed molecular weight by Western-blot: 39 kDa
  • Tested applications
    ELISA, IHC, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    35 beta calcimedin antibody; Annexin 4 antibody; annexin A4 antibody; Annexin IV antibody; ANX4 antibody; ANXA4 antibody; Chromobindin 4 antibody; DKFZp686H02120 antibody; Endonexin I antibody; LipOCortin IV antibody; P32.5 antibody; PAP II antibody; PIG28 antibody; PP4 X antibody; Protein II antibody; ZAP36 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Annexin IV recombinant protein (Accession Number: XM_047444081). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108095(Annexin IV Antibody) at dilution of 1:1000

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108095(Annexin IV Antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:108095(Annexin IV Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:108095(Annexin IV Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:108095(Annexin IV Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:108095(Annexin IV Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    Annexin IV /placental anticoagulant protein II (ANX4) is a member of the annexin or lipocortin family of calcium-dependent phospholipid binding proteins, and is located at 2p13. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 shares 45 to 59% identity with other members and possibly interacts with ATP. ANX4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. In western blotting, we get 36 kDa, 39 kDa(with modification), and 60 kDa( an unspecifc band).
  • References
    • Kulik L, Fleming SD, Moratz C. Pathogenic natural antibodies recognizing annexin IV are required to develop intestinal ischemia-reperfusion injury. Journal of immunology (Baltimore, Md. : 1950). 182(9):5363-73. 2009.
    • Rogers RS, Dharsee M, Ackloo S, Sivak JM, Flanagan JG. Proteomics analyses of human optic nerve head astrocytes following biomechanical strain. Molecular & cellular proteomics : MCP. 11(2):M111.012302. 2012.
    • Ying Q, Ansong E, Diamond AM, Lu Z, Yang W, Bie X. Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways. PloS one. 10(5):e0126285. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"