Product Name

Annexin A1 antibody

Documents

Description

Annexin A1 Rabbit Polyclonal antibody. Positive IHC detected in human oesophagus tissue, human kidney tissue, human lung tissue, human ovary tumor tissue, human small intestine tissue. Positive IF detected in A431 cells. Positive IP detected in HeLa cells. Positive WB detected in PC-3 cells, A431 cells, C6 cells, HeLa cells, HepG2 cells, K-562 cells, MCF7 cells, MDA-MB-453s cells, U-937 cells. Observed molecular weight by Western-blot: 37 kDa

Tested applications

ELISA, WB, IP, IHC, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

Annexin 1 antibody; annexin A1 antibody; Annexin I antibody; ANX1 antibody; ANXA1 antibody; Calpactin 2 antibody; Calpactin II antibody; Chromobindin 9 antibody; LipOCortin I antibody; LPC1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Annexin A1 recombinant protein (Accession Number: XM_011518609). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IP: 1:1000-1:10000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

WB result of Catalog No:108088(ANXA1 antibody).

Immunohistochemistry of paraffin-embedded human oesophagus tissue slide using Catalog No:108088(ANXA1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human oesophagus tissue slide using Catalog No:108088(ANXA1 Antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of A431 cells using Catalog No:108088(ANXA1 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108088(ANXA1 antibody) at dilution of 1:2000

IP Result of anti-ANXA1 (IP:Catalog No:108088, 3ug; Detection:Catalog No:108088 1:2000) with HeLa cells lysate 2400ug.

Background

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

References

Su TR, Lin JJ, Chiu CC. Proteomic investigation of anti-tumor activities exerted by sinularin against A2058 melanoma cells. Electrophoresis. 33(7):1139-52. 2012.
Chen RX, Song HY, Dong YY. Dynamic expression patterns of differential proteins during early invasion of hepatocellular carcinoma. PloS one. 9(3):e88543. 2014.
Tian H, Wang L, Cai R, Zheng L, Guo L. Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model. PloS one. 9(12):e116453. 2014.

Annexin A1 antibody

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