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Product Name
ANKRD53 antibody
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Description
ANKRD53 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human testis tissue. Observed molecular weight by Western-blot: 65-70 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ANKRD53 antibody; ankyrin repeat domain 53 antibody; FLJ12056 antibody; FLJ36160 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ANKRD53 recombinant protein (Accession Number: NM_024933). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108079(ANKRD53 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:108079(ANKRD53 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:108079(ANKRD53 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:108079(ANKRD53 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ANKRD53 also termed as ankyrin repeat domain 53 is a 533 amino-acid protein,which contains three ANK repeats and belongs to ankyrin family. The function of ANKRD53 is still non-known and need to be further studied. Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"