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Product Name
ANKRD27 antibody
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Description
ANKRD27 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in MCF-7 cells. Positive IP detected in A431 cells, MCF-7 cells. Observed molecular weight by Western-blot: 117-130 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human; other species not tested.
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Alternative names
ANKRD27 antibody; DKFZp434L0718 antibody; FLJ00040 antibody; PP12899 antibody; VARP antibody; VPS9 domain containing protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ANKRD27 recombinant protein (Accession Number: XM_006723412). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108073(ANKRD27 Antibody) at dilution of 1:1000
IP Result of anti-ANKRD27 (IP:Catalog No:108073, 4ug; Detection:Catalog No:108073 1:1000) with A431 cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:108073(ANKRD27 Antibody) at dilution of 1:50 (under 40x lens)
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Background
ANKRD27 represents ankyrin repeat domain 27 (VPS9 domain)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"