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Product Name
ANAPC5 Polyclonal Antibody
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Description
Polyclonal antibody to ANAPC5
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Tested applications
WB, IHC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
ANAPC5 antibody; APC5 antibody; anaphase-promoting complex subunit 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100 -
Validations
Western blot - ANAPC5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ANAPC5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ANAPC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using ANAPC5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ANAPC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using ANAPC5 antibody at dilution of 1:100 (40x lens).
Immunoprecipitation - ANAPC5 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug ANAPC5 antibody . Western blot was performed from the immunoprecipitate using ANAPC5 antibody at a dilition of 1:1000.
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Background
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
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