ANAPC2 Polyclonal Antibody

Cat.#: 165685

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Product Information

  • Product Name
    ANAPC2 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ANAPC2
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ANAPC2 antibody; APC2 antibody; anaphase-promoting complex subunit 2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human ANAPC2 (NP_037498.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - ANAPC2 Polyclonal Antibody

    Western blot - ANAPC2 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using ANAPC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.

  • Background
    Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"