Product Name

AMPK gamma2 antibody

Documents

Description

AMPK gamma2 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human heart tissue, human ovary tumor tissue. Positive IF detected in HeLa cells. Positive WB detected in mouse heart tissue, HEK-293 cells, HeLa cells, mouse eye tissue, PC-3 cells. Observed molecular weight by Western-blot: 63 kDa

Tested applications

ELISA, IHC, WB, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

AAKG antibody; AAKG2 antibody; AMPK gamma2 antibody; AMPK subunit gamma 2 antibody; CMH6 antibody; H91620p antibody; PRKAG2 antibody; WPWS antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of AMPK gamma2 recombinant protein (Accession Number: BC020540). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108011(PRKAG2 Antibody) at dilution of 1:600

Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:108011(PRKAG2 Antibody) at dilution of 1:50 (under 10x lens)

Immunofluorescent analysis of HeLa cells using Catalog No:108011(PRKAG2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

PRKAG2 represents protein kinase, AMP-activated, gamma 2 non-catalytic subunit

References

Zhang BL, Xu RL, Zhang J. Identification and functional analysis of a novel PRKAG2 mutation responsible for Chinese PRKAG2 cardiac syndrome reveal an important role of non-CBS domains in regulating the AMPK pathway. Journal of cardiology. 62(4):241-8. 2013.

AMPK gamma2 antibody

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