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Product Name
AMOT antibody
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Description
AMOT Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293. Observed molecular weight by Western-blot: 80 kDa; 130 kDa
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Tested applications
ELISA, IP, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
AMOT antibody; angiomotin antibody; KIAA1071 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AMOT recombinant protein (Accession Number: NM_133265). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: N/AIP: 1:200-1:1000
IF: 1:20-1:200
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Validations
WB results of Catalog No:107998 (1:1000) with over-expressed AMOT by Customer.
IP Result of anti-AMOT (IP:Catalog No:107998, 4ug; Detection:Catalog No:107998 1:300) with HEK-293 cells lysate 2500ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HEK-293 cells using Catalog No:107998(AMOT Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Angiomotin belongs to the motin family of angiostatin binding protein. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Most abundant expression was found in placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). This antibody recognizes both p130 and p80.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"