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Product Name
AMBRA1 antibody
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Description
AMBRA1 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, HepG2 cells, HT-1080 cells, human brain tissue, MCF7 cells. Positive IHC detected in human liver cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 130-135kd
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AMBRA1 antibody; autophagy/beclin 1 regulator 1 antibody; FLJ20294 antibody; KIAA1736 antibody; WDR94 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AMBRA1 recombinant protein (Accession Number: XM_005253014). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107988(AMBRA1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:107988(AMBRA1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107988(AMBRA1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application.
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