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Product Name
AMACR Polyclonal Antibody
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Description
Polyclonal antibody to AMACR
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
AMACR antibody; AMACRD antibody; CBAS4 antibody; P504S antibody; RACE antibody; RM antibody; alpha-methylacyl-CoA racemase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human AMACR (NP_976316.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - AMACR Polyclonal Antibody
Western blot analysis of extracts of mouse liver, using AMACR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
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Background
Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"