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alpha Tubulin antibody

Cat.#: 107874

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Product Information

  • Product Name
    alpha Tubulin antibody
  • Documents
  • Description
    alpha Tubulin Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in multi-cells/tissue, HeLa cells, mouse brain tissue, mouse colon tissue, mouse eye tissue, mouse liver tissue, mouse lung tissue, mouse uterus tissue, rat brain tissue. Positive IF detected in MCF-7 cells, HeLa cells. Positive IHC detected in human colon cancer tissue, human endometrial cancer tissue, human liver cancer tissue. Positive FC detected in HEK-293 cells. Observed molecular weight by Western-blot: 52kd
  • Tested applications
    ELISA, WB, IP, IHC, IF, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    alpha Tubulin antibody; Alpha tubulin ubiquitous antibody; alpha tubulin antibody; a tubulin antibody; TUBA1B antibody; tubulin antibody; tubulin antibody; alpha 1b antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of alpha Tubulin recombinant protein (Accession Number: NM_006082). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    Western blot on multiple cells with anti-Tubulin-Alpha (Catalog No:117297) at dilution 1:1,000.

    Western blot on multiple cells with anti-Tubulin-Alpha (Catalog No:117297) at dilution 1:1,000.

    Immunofluorescent analysis of MCF-7 cells using Catalog No:117297(Tubulin-Alpha Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Immunofluorescent analysis of MCF-7 cells using Catalog No:117297(Tubulin-Alpha Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Western blot of Hela cell with anti-Tubulin-Alpha (Catalog No:117297) at various dilutions.

    Western blot of Hela cell with anti-Tubulin-Alpha (Catalog No:117297) at various dilutions.

    1X10^6 HEK-293 cells were stained with 0.2ug Tubulin-Alpha antibody (Catalog No:117297, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

    1X10^6 HEK-293 cells were stained with 0.2ug Tubulin-Alpha antibody (Catalog No:117297, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:117297(Tubulin-Alpha antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:117297(Tubulin-Alpha antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:117297(Tubulin-Alpha antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:117297(Tubulin-Alpha antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-Tubulin-Alpha (IP:Catalog No:117297, 3ug; Detection:Catalog No:117297 1:1000) with HeLa cells lysate 5000ug.

    IP Result of anti-Tubulin-Alpha (IP:Catalog No:117297, 3ug; Detection:Catalog No:117297 1:1000) with HeLa cells lysate 5000ug.

  • Background
    There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
  • References
    • MacFarlane LA, Gu Y, Casson AG, Murphy PR. Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2. Molecular endocrinology (Baltimore, Md.). 24(4):800-12. 2010.
    • MacFarlane LA, Murphy PR. Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression. Molecular carcinogenesis. 49(12):1031-44. 2010.
    • Thakar K, Karaca S, Port SA, Urlaub H, Kehlenbach RH. Identification of CRM1-dependent Nuclear Export Cargos Using Quantitative Mass Spectrometry. Molecular & cellular proteomics : MCP. 12(3):664-78. 2013.
    • Roloff S, Spillner C, Kehlenbach RH. Several phenylalanine-glycine motives in the nucleoporin Nup214 are essential for binding of the nuclear export receptor CRM1. The Journal of biological chemistry. 288(6):3952-63. 2013.
    • Poolman TM, Farrow SN, Matthews L, Loudon AS, Ray DW. Pin1 promotes GR transactivation by enhancing recruitment to target genes. Nucleic acids research. 41(18):8515-25. 2013.
    • Schulz O, Pieper C, Clever M. Resolution doubling in fluorescence microscopy with confocal spinning-disk image scanning microscopy. Proceedings of the National Academy of Sciences of the United States of America. 110(52):21000-5. 2013.
    • McEachern LA, Murphy PR. Chromatin-remodeling factors mediate the balance of sense-antisense transcription at the FGF2 locus. Molecular endocrinology (Baltimore, Md.). 28(4):477-89. 2014.
    • Frohnert C, Hutten S, Wälde S, Nath A, Kehlenbach RH. Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase. PloS one. 9(2):e88887. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"