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Product Name
alpha Tubulin antibody
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Description
alpha Tubulin Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue, human brain tissue, human kidney tissue, human medulloblastoma tissue, human ovary tissue, human placenta tissue, human skin tissue. Positive WB detected in HeLa cells, mouse cerebellum tissue. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
alpha tubulin antibody; TUBA1A antibody; TUBA3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of alpha Tubulin recombinant protein (Accession Number: NM_006009). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117300(tubulin-Alpha antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:117300(tubulin-Alpha antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:117300(tubulin-Alpha antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:117300(tubulin-Alpha Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
TUBA1A represents tubulin, alpha 1a
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References
- Shen E, Lei Y, Liu Q. Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression. Experimental cell research. 315(7):1100-16. 2009.
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