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Product Name
Alpha E catenin antibody
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Description
Alpha E catenin Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse uterus tissue. Positive IHC detected in human stomach tissue, human colon tissue, human pancreas cancer tissue. Positive IF detected in A431 cells. Positive FC detected in MCF-7 cells. Observed molecular weight by Western-blot: 100 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
alpha catenin antibody; Alpha E catenin antibody; Cadherin assOCiated protein antibody; CAP102 antibody; Catenin alpha 1 antibody; CTNNA1 antibody; α-catenin antibody; α-E-catenin antibody; αE-catenin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Alpha E catenin recombinant protein (Accession Number: NM_001324000). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:107869(CTNNA1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A431 cells using Catalog No:107869(CTNNA1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
1X10^6 MCF-7 cells were stained with 0.2ug CTNNA1 antibody (Catalog No:107869, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
mouse uterus tissue were subjected to SDS PAGE followed by western blot with Catalog No:107869(CTNNA1 Antibody) at dilution of 1:2000
IP Result of anti-CTNNA1 (IP:Catalog No:107869, 4ug; Detection:Catalog No:107869 1:700) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:107869(CTNNA1 Antibody) at dilution of 1:50 (under 10x lens)
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Background
Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.
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References
- Zhang C, Zhang H, Yu L, Cao Y. Helicobacter pylori dwelling on the apical surface of gastrointestinal epithelium damages the mucosal barrier through direct contact. Helicobacter. 19(5):330-42. 2014.
- Zhang Y, Zhang X, Huang J, Dong Q. Wnt signaling regulation of stem-like properties in human lung adenocarcinoma cell lines. Medical oncology (Northwood, London, England). 32(5):157. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"