alpha-Defensin-6 (Human) peptide

alpha-Defensin-6 (Human) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-AFTCHCRRSCYSTEYSYGTCTVMGINHRFCCL-OH(Disulfide bonds between Cys4- Cys31, Cys6- Cys20, and Cys10- Cys30)

H-Ala-Phe-Thr-Cys-His-Cys-Arg-Arg-Ser-Cys-Tyr-Ser-Thr-Glu-Tyr-Ser-Tyr-Gly-Thr-Cys-Thr-Val-Met-Gly-Ile-Asn-His-Arg-Phe-Cys-Cys-Leu-OH

32

95.2%

C₁₅₆H₂₂₈N₄₆O₄₆S₇

3708.2

Synthetic

Powder

Cysteine-rich cationic peptide with antiviral activity against HSV infection and a role in N. gonorrhoeae-mediated HIV enhancement. In contrast to the other members of the ƒ-defensin family it shows very low antimicrobial activity against Gram-negative and Gram-positive bacteria. As a biomarker of colon adenocarcinoma it may have utility in colon cancer screening and diagnosis.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Z.Wu et al., J. Pept. Res., 64, 118 (2004)
• M.J.Nam et al., J. Biol. Chem., 280, 8260 (2005)
• E.Hazrati et al., J. Immunol., 177, 8658 (2006)
• A.Szyk et al., Protein Sci., 15, 2749 (2006)
• M.E.Klotman et al., J. Immunol., 180, 6176 (2008)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.