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Product Name
alpha cardiac Actin-specific antibody
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Description
alpha cardiac Actin-specific Mouse Monoclonal antibody. Positive IHC detected in human heart tissue, human heart and human skeletal muscle tissue. Positive WB detected in human heart tissue. Observed molecular weight by Western-blot: 42 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
ACTC antibody; ACTC1 antibody; ACTC1 cardiac muscle 1 antibody; ACTC1 specific antibody; Actin cardiac muscle specific antibody; Actin antibody; alpha cardiac muscle 1 antibody; actin antibody; alpha antibody; cardiac muscle 1 antibody; Alpha cardiac actin antibody; CMD1R antibody; CMH11 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005159). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:20000
IHC: 1:100-1:400
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Validations
Immunohistochemistry of paraffin-embedded human heart and human skeletal muscle using Catalog No:107575 (ACTC1 specific antibody) at dilution 1:500. (under 40x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:107575(Actin-cardiac-muscle-1-Specific antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:107575(Actin-cardiac-muscle-1-Specific antibody) at dilution of 1:200 (under 40x lens)
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107575(Actin-cardiac-muscle-1-Specific antibody) at dilution of 1:5000
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Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.
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