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Product Name
alpha Adaptin antibody
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Description
alpha Adaptin Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive IF detected in HEK-293 cells. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in HEK-293 cells, human heart tissue, human spleen tissue, mouse spleen tissue. Observed molecular weight by Western-blot: 108 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADTAA antibody; alpha Adaptin antibody; Alpha adaptin A antibody; Alpha1 adaptin antibody; AP 2 complex subunit alpha 1 antibody; AP2 ALPHA antibody; AP2A1 antibody; CLAPA1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of alpha Adaptin recombinant protein (Accession Number: BC014214). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107866(AP2A1 antibody) at dilution of 1:1000
IP Result of anti-AP2A1 (IP:Catalog No:107866, 3ug; Detection:Catalog No:107866 1:1000) with mouse skeletal muscle tissue lysate 3500ug.
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:107866(AP2A1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:107866(AP2A1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.
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References
- Purdy AK, Alvarez Arias DA, Oshinsky J, James AM, Serebriiskii I, Campbell KS. The ap-2 clathrin adaptor mediates endocytosis of an inhibitory killer cell Ig-like receptor in human NK cells. Journal of immunology (Baltimore, Md. : 1950). 193(9):4675-83. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"