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Product Name
Human MGAT5 (His Tag) recombinant protein
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Description
Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
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Protein name
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
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Protein short names
4930471A21RIK; MGAT5; 5330407H02RIK; GNT-V; GNT-VA; GLCNAC-TV; AI480971
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Uniprot ID
Q09328
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Gene Name
MGAT5; GGNT5
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human MGAT5 (NP_002401.1) C-terminal segment (Leu 189-Leu 741) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the terminus.
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Protein Species
Human
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Molecular weight
The recombinant human MGAT5 consists of 564 amino acids and predictes a molecular mass of 65 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MGAT5 is approximately 60-65 kDa.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
Measured by its ability to transfer NAcetylαDglucosamine from UDPNAcetylαDglucosamine to a biantennary Nlinked core pentasaccharide in a CD39L3 coupled assay. The specific activity is >10pmoles/min/μg
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Validations
Human MGAT5 / GGNT5 Protein (His Tag) SDS-PAGE
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