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Product Name
ALG2 Polyclonal Antibody
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Description
Polyclonal antibody to ALG2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
ALG2 antibody; CDG1I antibody; CDGIi antibody; CMS14 antibody; CMSTA3 antibody; NET38 antibody; hALPG2 antibody; alpha-1,3/1,6-mannosyltransferase ALG2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 197-416 of human ALG2 (NP_149078.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - ALG2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ALG2 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit ._Exposure time: 90s.
Immunohistochemistry - ALG2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using ALG2 Antibody at dilution of 1:100 (40x lens).
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Background
Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"