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Product Name
ALG1 antibody
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Description
ALG1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 47-53 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALG1 antibody; Beta 1 antibody; 4 mannosyltransferase antibody; hMat 1 antibody; HMAT1 antibody; HMT 1 antibody; HMT1 antibody; Mannosyltransferase 1 antibody; MT 1 antibody; UNQ861/PRO1870 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ALG1 recombinant protein (Accession Number: NM_019109). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107979(ALG1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:107979(ALG1 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:107979(ALG1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ALG1 represents asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)
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References
- Isono T. O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation. PloS one. 6(4):e18959. 2011.
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