ALG1 antibody

Cat.#: 107979

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Product Information

  • Product Name
    ALG1 antibody
  • Documents
  • Description
    ALG1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 47-53 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ALG1 antibody; Beta 1 antibody; 4 mannosyltransferase antibody; hMat 1 antibody; HMAT1 antibody; HMT 1 antibody; HMT1 antibody; Mannosyltransferase 1 antibody; MT 1 antibody; UNQ861/PRO1870 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ALG1 recombinant protein (Accession Number: NM_019109). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107979(ALG1 antibody) at dilution of 1:300

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107979(ALG1 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:107979(ALG1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:107979(ALG1 antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:107979(ALG1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:107979(ALG1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    ALG1 represents asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)
  • References
    • Isono T. O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation. PloS one. 6(4):e18959. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"