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Product Name
ALDH4A1 antibody
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Description
ALDH4A1 Rabbit Polyclonal antibody. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in human heart tissue, HepG2 cells, human brain tissue, human liver tissue, human skeletal muscle tissue, K-562 cells, mouse skeletal muscle tissue, mouse small intestine tissue, rat heart tissue. Positive IHC detected in human liver tissue, human prostate cancer tissue. Observed molecular weight by Western-blot: 62kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALDH4 antibody; ALDH4A1 antibody; P5C dehydrogenase antibody; P5CD antibody; P5CDh antibody; P5CDhL antibody; P5CDhS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ALDH4A1 recombinant protein (Accession Number: NM_003748). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107971(ALDH4A1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human liver using Catalog No:107971(ALDH4A1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107971(ALDH4A1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ALDH4A1 (IP:Catalog No:107971, 4ug; Detection:Catalog No:107971 1:800) with mouse skeletal muscle tissue lysate 3600ug.
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Background
ALDH4A1(Aldehyde dehydrogenase family 4 member A1) is also named as ALDH4, P5CDH,P5C dehydrogenase and belongs to the aldehyde dehydrogenase family. P5CDH is a mitochondrial matrix NAD(+)-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate.Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2).
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References
- Liu W, Le A, Hancock C. Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC. Proceedings of the National Academy of Sciences of the United States of America. 109(23):8983-8. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"