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Product Name
ALDH2 antibody
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Description
ALDH2 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human heart tissue. Positive IP detected in mouse lung tissue. Positive WB detected in mouse lung tissue, human heart tissue, human kidney tissue, human liver tissue, MCF7 cells, mouse skeletal muscle tissue, rat lung tissue. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALDH class 2 antibody; ALDH E2 antibody; ALDH2 antibody; ALDHI antibody; ALDM antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ALDH2 recombinant protein (Accession Number: NM_000690). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:107966(ALDH2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:107966(ALDH2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107966(ALDH2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ALDH2 (IP:Catalog No:107966, 4ug; Detection:Catalog No:107966 1:1000) with mouse lung tissue lysate 2800ug.
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Background
Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.
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References
- Amanuma Y, Ohashi S, Itatani Y. Protective role of ALDH2 against acetaldehyde-derived DNA damage in oesophageal squamous epithelium. Scientific reports. 5:14142. 2015.
- Oberbeck N, Langevin F, King G, de Wind N, Crossan GP, Patel KJ. Maternal aldehyde elimination during pregnancy preserves the fetal genome. Molecular cell. 55(6):807-17. 2014.
- Ying Q, Ansong E, Diamond AM, Lu Z, Yang W, Bie X. Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways. PloS one. 10(5):e0126285. 2015.
- Liu H, Zhang H, Cui R, Guo X, Wang D, Dai J. Activation of peroxisome proliferator-activated receptor α ameliorates perfluorododecanoic acid-induced production of reactive oxygen species in rat liver. Archives of toxicology. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"