-
Product Name
ALDH1A2 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to ALDH1A2
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
ALDH1A2 antibody; RALDH antibody; RALDH2 antibody; RALDH2-T antibody; retinal dehydrogenase 2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALDH1A2 (NP_733797.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ALDH1A2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ALDH1A2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ALDH1A2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using ALDH1A2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - ALDH1A2 Polyclonal Antibody
Immunofluorescence analysis of C6 cells using ALDH1A2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - ALDH1A2 Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using ALDH1A2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"