Product Name

ALDH1A1 antibody

Documents

Description

ALDH1A1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human eye tissue, human liver tissue, human testis tissue, mouse testis tissue. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, HL-60 cells, human liver tissue, K-562 cells. Observed molecular weight by Western-blot: 50-55 kDa

Tested applications

ELISA, WB, IHC, IP, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

ALDC antibody; ALDH E1 antibody; ALDH1 antibody; ALDH11 antibody; ALDH1A1 antibody; ALHDII antibody; PUMB1 antibody; RALDH 1 antibody; RALDH1 antibody; Retinal dehydrogenase 1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of ALDH1A1 recombinant protein (Accession Number: NM_000689). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:107958(ALDH1A1 antibody) at dilution of 1:400

Immunohistochemical of paraffin-embedded human eye using Catalog No:107958(ALDH1A1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human eye using Catalog No:107958(ALDH1A1 antibody) at dilution of 1:100 (under 40x lens)

IP Result of anti-ALDH1A1 (IP:Catalog No:107958, 4ug; Detection:Catalog No:107958 1:500) with mouse liver tissue lysate 6400ug.

Immunofluorescent analysis of HepG2 cells using Catalog No:107958(ALDH1A1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).

References

Yan D, Geusz ME, Jamasbi RJ. Properties of lewis lung carcinoma cells surviving curcumin toxicity. Journal of Cancer. 3:32-41. 2012.
Almanaa TN, Geusz ME, Jamasbi RJ. Effects of curcumin on stem-like cells in human esophageal squamous carcinoma cell lines. BMC complementary and alternative medicine. 12:195. 2012.
Huang CF, Xu XR, Wu TF, Sun ZJ, Zhang WF. Correlation of ALDH1, CD44, OCT4 and SOX2 in tongue squamous cell carcinoma and their association with disease progression and prognosis. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 43(7):492-8. 2014.
He KF, Zhang L, Huang CF. CD163+ tumor-associated macrophages correlated with poor prognosis and cancer stem cells in oral squamous cell carcinoma. BioMed research international. 2014:838632. 2014.
Oberbeck N, Langevin F, King G, de Wind N, Crossan GP, Patel KJ. Maternal aldehyde elimination during pregnancy preserves the fetal genome. Molecular cell. 55(6):807-17. 2014.
Ma SR, Wang WM, Huang CF, Zhang WF, Sun ZJ. Anterior gradient protein 2 expression in high grade head and neck squamous cell carcinoma correlated with cancer stem cell and epithelial mesenchymal transition. Oncotarget. 6(11):8807-21. 2015.

ALDH1A1 antibody

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