-
Product Name
ALAD Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to ALAD
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human
-
Alternative names
ALAD antibody; ALADH antibody; PBGS antibody; aminolevulinate dehydratase antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ALAD (NP_000022.3).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ALAD Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ALAD antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ALAD Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using ALAD antibody at dilution of 1:100 (40x lens).
-
Background
Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"