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Product Name
AKTIP Polyclonal Antibody
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Description
Polyclonal antibody to AKTIP
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
AKTIP antibody; FT1 antibody; FTS antibody; AKT-interacting protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human AKTIP (NP_071921.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - AKTIP Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AKTIP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 40s.
Immunohistochemistry - AKTIP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using AKTIP antibody at dilution of 1:100 (40x lens).
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Background
Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"