-
Product Name
AKR1C2 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to AKR1C2
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse
-
Alternative names
AKR1C2 antibody; AKR1C-pseudo antibody; BABP antibody; DD antibody; DD-2 antibody; DD/BABP antibody; DD2 antibody; DDH2 antibody; HAKRD antibody; HBAB antibody; MCDR2 antibody; SRXY8 antibody; TDD antibody; aldo-keto reductase family 1 member C2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C2 (NP_995317.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - AKR1C2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AKR1C2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
-
Background
Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"