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Product Name
AKNA antibody
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Description
AKNA Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue, human spleen tissue. Positive WB detected in Raji cells, K-562 cells, U-937 cells. Observed molecular weight by Western-blot: 65-70 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
AKNA antibody; AT hook transcription factor antibody; FLJ31001 antibody; FLJ33184 antibody; KIAA1968 antibody; RP11 82I1.4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AKNA recombinant protein (Accession Number: XM_047423921). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:107937(AKNA Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107937(AKNA Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:107937(AKNA Antibody) at dilution of 1:25 (under 40x lens)
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Background
AKNA also named as AT hook transcription factor is a 1439 amino-acids protein. AKNA, encodes a lymphoid-specific AT-hook transcription factor that is highly expressed by B and T lymphocytes. It coordinately regulates expression of the CD40 receptor and its ligand (CD154), two molecules that are critical for antigen-dependent B-cell development. AKNA expresses at least nine distinct transcripts, some of which are expressed in a tissue-specific manner. These transcripts are products of alternative splicing, alternative promoter usage, and alternative polyadenylation. The alternative AKNA transcripts are predicted to encode overlapping protein isoforms, some of which (p70 and p100) (PMID:15869410)
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