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Product Name
AKAP5 Polyclonal Antibody
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Description
Polyclonal antibody to AKAP5
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
AKAP5 antibody; AKAP75 antibody; AKAP79 antibody; H21 antibody; A-kinase anchor protein 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human AKAP5 (NP_004848.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - AKAP5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AKAP5 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - AKAP5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using AKAP5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - AKAP5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using AKAP5 antibody at dilution of 1:100 (40x lens).
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Background
May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"