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Product Name
AKAP4 Polyclonal Antibody
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Description
Polyclonal antibody to AKAP4
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Tested applications
WB
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Species reactivity
Mouse, Rat
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Alternative names
AKAP4 antibody; AKAP 82 antibody; AKAP-4 antibody; AKAP82 antibody; CT99 antibody; FSC1 antibody; HI antibody; PRKA4 antibody; hAKAP82 antibody; p82 antibody; A-kinase anchor protein 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 189-340 of human AKAP4 (NP_003877.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - AKAP4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AKAP4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 3s.
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Background
Major structural component of sperm fibrous sheath. Plays a role in sperm motility.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"