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Product Name
AKAP1 antibody
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Description
AKAP1 Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human colon cancer tissue, human prostate cancer tissue, human small intestine tissue. Positive IP detected in PC-3 cells. Positive WB detected in HeLa cells, human liver tissue, PC-3 cells. Observed molecular weight by Western-blot: 149 kDa
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Tested applications
ELISA, WB, IF, IHC, FC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
AKAP antibody; AKAP 149 antibody; AKAP1 antibody; AKAP121 antibody; AKAP149 antibody; AKAP84 antibody; D AKAP 1 antibody; D AKAP1 antibody; PRKA1 antibody; S AKAP84 antibody; SAKAP84 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AKAP1 recombinant protein (Accession Number: XM_005257709). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107927(AKAP1 antibody) at dilution of 1:1000
Immunofluorescent analysis of MCF-7 cells, using AKAP1 antibody Catalog No:107927 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-AKAP1 (IP:Catalog No:107927, 4ug; Detection:Catalog No:107927 1:2000) with PC-3 cells lysate 1800ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:107927(AKAP1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:107927(AKAP1 Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 MCF-7 cells were stained with 0.2ug AKAP1 antibody (Catalog No:107927, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.
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References
- Grozdanov PN, Stocco DM. Short RNA molecules with high binding affinity to the KH motif of A-kinase anchoring protein 1 (AKAP1): implications for the regulation of steroidogenesis. Molecular endocrinology (Baltimore, Md.). 26(12):2104-17. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"