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Product Name
AIM2 antibody
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Description
AIM2 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, A549 cells, COLO 320 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF7 cells, SH-SY5Y cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 40-45kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
absent in melanoma 2 antibody; AIM2 antibody; PYHIN4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AIM2 recombinant protein (Accession Number: BC010940). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107855(AIM2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:107855(AIM2 antibody) at dilution of 1:100 (under 10x lens)
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Background
Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831].
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References
- Wu DL, Xu GH, Lu SM. Correlation of AIM2 expression in peripheral blood mononuclear cells from humans with acute and chronic hepatitis B. Human immunology. 74(5):514-21. 2013.
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