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Product Name
AHCYL1 antibody
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Description
AHCYL1 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in Jurkat cells, HeLa cells, mouse thymus tissue. Observed molecular weight by Western-blot: 60kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AdoHcyase 2 antibody; AHCYL1 antibody; DCAL antibody; IRBIT antibody; PRO0233 antibody; XPVKONA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AHCYL1 recombinant protein (Accession Number: NM_006621). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107843(AHCYL1 antibody) at dilution of 1:1000
Immunofluorescent analysis of Hela cells, using AHCYL1 antibody Catalog No:107843 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor-binding protein released with IP3 (IRBIT), regulates IP3-induced Ca2+ release into the cytoplasm of cells(PMID:23145124). This protein is also named as DCAL, XPVKONA and belongs to the adenosylhomocysteinase family. AHCYL1 mRNA and protein are most abundant in the glandular epithelium of adenocarcinoma of cancerous, but not normal and AHCYL1 protein is predominantly in the nucleus(PMID:22826361). It has 2 isoforms produced by alternative splicing.
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References
- Li X, Sun Q, Li X. Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions. European journal of nutrition. 54(7):1201-10. 2015.
- Hu Y, Sun Q, Li X. In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks. PloS one. 10(4):e0122643. 2015.
- Jia Y, Song H, Gao G, Cai D, Yang X, Zhao R. Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets. Journal of agricultural and food chemistry. 63(46):10152-60. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"