-
Product Name
AHCY antibody
- Documents
-
Description
AHCY Mouse Monoclonal antibody. Positive WB detected in HeLa cells, human testis tissue, L02 cells, MCF7 cells. Positive IHC detected in human testis tissue, human placenta tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 48kd
-
Tested applications
ELISA, IHC, IF, WB
-
Species reactivity
Human; other species not tested.
-
Alternative names
AdenosylhomOCysteinase antibody; AdoHcyase antibody; AHCY antibody; S adenosylhomOCysteine hydrolase antibody; SAHH antibody
-
Isotype
Mouse IgG2b
-
Preparation
This antibody was obtained by immunization of AHCY recombinant protein (Accession Number: NM_001362750). Purification method: Protein A purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107563(AHCY antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:107563(AHCY antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:107563(AHCY antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using AHCY antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
-
Background
AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"