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Product Name
AGPAT2 Polyclonal Antibody
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Description
Polyclonal antibody to AGPAT2
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
AGPAT2 antibody; 1-AGPAT2 antibody; BSCL antibody; BSCL1 antibody; LPAAB antibody; LPAAT-beta antibody; 1-acylglycerol-3-phosphate O-acyltransferase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 199-278 of human AGPAT2 (NP_006403.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - AGPAT2 Polyclonal Antibody
Western blot analysis of extracts of HT-29 cells, using AGPAT2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - AGPAT2 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using AGPAT2 antibody . Blue: DAPI for nuclear staining.
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Background
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"