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Product Name
AGPAT1 Polyclonal Antibody
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Description
Polyclonal antibody to AGPAT1
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
AGPAT1 antibody; 1-AGPAT1 antibody; G15 antibody; LPAAT-alpha antibody; LPAATA antibody; 1-acylglycerol-3-phosphate O-acyltransferase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 204-283 of human AGPAT1 (NP_006402.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:50 - 1:100 -
Validations
Western blot - AGPAT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AGPAT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunoprecipitation - AGPAT1 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug AGPAT1 antibody . Western blot was performed from the immunoprecipitate using AGPAT1 antibody at a dilition of 1:1000.
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Background
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"