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Product Name
AGAP2 antibody
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Description
AGAP2 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, rat brain tissue. Positive IHC detected in human gliomas tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 124 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AGAP 2 antibody; AGAP2 antibody; Centaurin gamma 1 antibody; CENTG1 antibody; Cnt g1 antibody; FLJ16430 antibody; GGAP2 antibody; KIAA0167 antibody; PIKE antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AGAP2 recombinant protein (Accession Number: NM_014770). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107914(AGAP2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:107914(AGAP2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:107914(AGAP2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using AGAP2 antibody Catalog No:107914 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-AGAP2 (IP:Catalog No:107914, 4ug; Detection:Catalog No:107914 1:500) with mouse brain tissue lysate 4000ug.
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Background
AGAP2 represents ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
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