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Product Name
AES Polyclonal Antibody
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Description
Polyclonal antibody to AES
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
AES antibody; AES-1 antibody; AES-2 antibody; ESP1 antibody; GRG antibody; GRG5 antibody; Grg-5 antibody; TLE5 antibody; amino-terminal enhancer of split antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human AES (NP_945320.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - AES Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AES antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - AES Polyclonal Antibody
Immunofluorescence analysis of A549 cells using AES antibody . Blue: DAPI for nuclear staining.
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Background
Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"